Biological reduction of nitrate wastewater using a fluidized-bed bioreactor

"There are a number of nitrate-containing wastewater sources, as concentrated as 30 wt % NO3 and as large as 2000 m3/day, in the nuclear fuel cycle. The biological reduction of nitrate in waste water to gaseous nitrogen, accompanied by the oxidation of a nutrient carbon source to gaseous carbon dioxide, is an ecologically sound and cost-effective method of nitrate waste disposal. These nitrate-containing wastewater sources can be successfully biologically denitrified to meet discharge standards in the range of 10 to 20 g N(NO3-)/m3 by the use of a fluidized bed bioreactor. The denitrification bacteria are a mixed culture derived from garden soil; the major strain is Pseudomonas. In the fluidized-bed bioreactor, the bacteria are allowed to attach to 0.25- to 0.50-mm-diam. coal fluidization particles, which are then fluidized by the upward flow of influent wastewater. Maintaining the bacteria-to-coal weight ratio at approximately 1:10 results in a bioreactor bacteria loading of greater than 20,000 g/m3,This paper describes the results of a bio denitrification R&D program based on the use of fluidized bioreactors capable of operating at nitrate levels of up to 7000 g/m3 and achieving denitrification rates as high as 80 g N(N03-) per day per litre of empty bioreactor volume. IntroductionThe discharge of wastewater containing nitrate at concentrations greater than stipulated by the Environmental Protection Agency (EPA) creates an environmental hazard and constitutes a civil and/ or criminal legal liability.(I.2) At present, we have three options: (1) recycle and reuse of nitrate streams the total economic and technical balance should be Closely examined; (2) use of an alternative acid, which may pose an entirely different set of problems ; or (3) disposal of nonreusable nitrate sources by an environmentally acceptable procedure."