Comparison of Adhesion of A. ferrooxidans on Different Copper Sulfides: Surface Thermodynamics and Extended DLVO Theory

"We investigated experimentally and theoretically the adhesion behavior of Acidithiobacillus ferrooxidans (At.f6) to different copper sulfides. The adsorption experiment showed cell densities of 10.74 × 106, 8.06 × 106, 3 × 106, 4.24 × 106 and 2.35 × 106 cells/cm2, respectively, on the surfaces of djurleite, bornite, covellite and chalcopyrite. The order was: djurleite > bornite > covellite > chalcopyrite. The results indicate that A. ferrooxidans has high affinity to adhering to the surfaces of djurleite and bornite in the acidic region. But the thermodynamic approach predicts that there will be no attachment of At.f6 to the surfaces of bornite, covellite and chalcopyrite. This discrepancy is due to the inadequate description of electrostatic interactions. The DLVO approach shows attachment of At.f6 to the surfaces of djurleite, bornite and covellite, and repulsion between At.f6 and chalcopyrite. The DLVO approach can explain the differences of the adhesion of At.f6 to different copper sulfides.IntroductionThe bioleaching of low-grade copper sulfides is a developing technology, and has been applied successfully to the extraction of copper from secondary sulfide minerals (Watling, 2006; Pradhan et al., 2008; Akcil and Deveci, 2010). The bioleaching of chalcopyrite is still a major challenge (Bosecker, 1997; Brierley and Brierley, 2001; Ehrlich, 2004). A study conducted at Billiton Process Research showed the following preferential order of sulfide minerals leached by mesophilic cultures: chalcocite > bornite > cubanite > covellite > pyrite > enargite > carrolite >> chalcopyrite (Dew et al., 1999). The bacteria can solubilize the valuable metals from the orebodies by the oxidation of minerals. Bacterial adhesion plays a critical role in the bioleaching processes (Chen et al., 2008; Harneit et al., 2006). Generally, bacterial adhesion can be explained by surface thermodynamics and the extended DLVO theory, in which the adhesion energy between the bacteria and mineral is calculated as a function of the separation distance (Sharma, 1999; Bos et al., 1999; Sharma and Hanumantha Rao, 2002). These methods take into account Lifshitz-van der Waals interactions, electrostatic interactions and acid-base interactions (Busscher et al., 2010). These interactions are very well understood and formulated in mathematical equations. The most important inputs in these calculations are surface energy, surface charge, electron donation and electron acceptance on the bacterial cell surface and mineral."