High Gradient Magnetic Separation Of Red Blood Cells: Current Status.

Several attempts have been made over the past 40 years to separate red blood cells from whole blood using a magnet. This has been achieved by the authors by using a high gradient magnetic field and " carrying " the red cells in a suitable reducing agent - the deoxyhaemoglobin so produced has 4 unpaired electrons per atom giving appreciable paramagnetic moment as opposed to the usual oxygenated form which is diamagnetic. Red blood cells so separated appear to be undamaged and can be reinjected into a donor animal where their survival is shown to be normal using an isotope tagging technique. Studies on function of the white blood cells and blood platelets also indicate that they are functionally unchanged by separation process.