Mechanism of Adhesion of Bacillus subtilis onto Dolomite

The adhesion (and de-adhesion) of Bacillus subtilis to dolomite was determined under a number of conditions of initial concentration of the organism, agitation, mineral particle size and temperature. Rate equations curves for both adhesion and de-adhesion were constructed, with the rates of de-adhesion being about half that of adhesion. From the temperature data the energy of adhesion was determined to be about 1.02 kcal/mol. Adhesion of dolomite to B. subtilis follows a Langmuir isotherm. Equilibrium constants, adhesion capacity and Gibbs free energy of adhesion of B. subtilis to dolomite (at pH 6.6) were determined. These values were compared to similar data for adhesion of B. subtilis to apatite and dolomite.