The Effects Of Dipalmitoyl Lecithin Coated Fresh Quartz On Superoxide Release From Alveolar Macrophages In Vitro

The hypothesis that freshly-sheared quartz makes it more reactive in lung tissue than aged quartz was tested by its ability to stimulate superoxide anion (02-) release from single adherent pulmonary alveolar macrophages (PAM). Fresh quartz was prepared by grinding for 30 min and then used within 2 hr, while stale quartz was from the same stock but aged 15 days prior to use. Also, dipalmitoyl lecithin (DPL), the major constituent of lung surfactant coating the alveoli surfaces, was coated onto the particles. Release of 02- was measured after 40 and 180 min of direct contact with quartz dust (225 µg/ml). Fresh quartz was more reactive than aged quartz, as indicated by the 78 and 63% increase after 40 and 180 min, respectively. A time delay and suppression of 02- release was observed in the ability of the DPL- coated fresh or stale dust to stimulate PAM. These results supported the hypothesis that the natural process of surfactant coating of inhaled particles in the alveoli may play a role in their phagocytic removal by PAM.