Toxicity and Metal Speciation in Acid Mine Drainage Treated by Passive Bioreactors

Sulfate-reducing passive bioreactors efficiently treat acid mine drainage (AMD) by increasing its pH and alkalinity and by removing metals as sulfide precipitates, in addition to hydroxides and carbonates. However, the new mining legislation sets discharge limits based on physicochemical parameters and on the non-toxicity of treated effluent as well. In this study, toxicity was assessed for effluent from 3.5 L column bioreactors filled with mixtures of natural organic carbon sources and operated at two different hydraulic retention times (HRTs) of 7.3 d and 10 d for the treatment of a highly contaminated AMD. Acute toxicity was observed for D. magna, and a toxicity identification evaluation (TIE) procedure was performed to identify potential toxicants. To help the interpretation of the toxicity results, metal partition in the effluent was also determined by filtration and ultrafiltration, while geochemical modeling was performed for metal speciation evaluation. The 10 d HRT effluent was nonacutely lethal for rainbow trout but acutely lethal for D. magna, whereas the 7.3 d HRT effluent was toxic for both organisms. The toxicity to D. magna was eliminated by 2 h of aeration for the 10 d HRT effluent, and the TIE procedure suggested iron as a cause of toxicity. However, the 2 h aeration eliminated the toxicity of the 7.3 d HRT effluent, only if performed after the pH rising to 9.3. Data regarding metal speciation indicated instability of both effluents during aeration and were consistent with the toxicity being caused by iron. The study indicates that design of passive treatment should include sufficient HRT and posttreatment aeration to meet acute toxicity requirements. Keywords: acid mine drainage, sulphate-reducing passive bioreactors, hydraulic retention time, effluent toxicity, metal speciation