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By Carlos Del Castillo Whittembury

El presente texto señala el uso de la bioRemediación como principal técnica que permite la digestión de residuos orgánicos contaminantes de aguas, suelos y aires. En el caso del agua, su limitación está en el tiempo de mantenimiento (5 días) donde se necesita contar con tanques o pozas acordes con los flujos a tratar, así como el control periódico del sistema. sistema. En el caso de suelos contaminados, es una de las opciones más convenientes por la facilidad de aplicación y la capacidad del tratamiento para remediar. Finalmente, en el uso del aire, los sopladores con membranas porosas mejoran los niveles de oxígeno disuelto, empleando menor energía eléctrica, cuyo aspecto redunda en un menor costo de los tratamientos. En este sentido, se busca mostrar nuevas tendencias de tratamientos a fin de regresar a las condiciones originales las aguas, suelos y aires contaminados por residuos orgánicos, inorgánicos e incluso por hidrocarburos, las cuales están disponibles en el Perú.

Sep 12, 2005

By Gh. Crutu

During exploration and exploitation of complex ores, waste dumps that contaminate severely the environment and in particular soils and waters, are genetated. The most hazardous pollutants are heavy metals and metalloids (e.g As, Mo, Pb and Cu) as well as radionuclides (e.g U and Ra).

Jan 1, 2008

By M. Christou

The LIFE-Environment Demonstration Project ?Treating Waste for Restoring Land Sustainability?(www.bangor.ac.uk/ies/TWIRLS/TWIRLS_home.htm), a project initiated by the University of Wales, Bangor, UK in which NAGREF is a partner, aims to demonstrate the effective use of industrial and municipal wastes for the remediation of post-industrial and degraded sites in urban and rural sites. Within the framework of this project, a schist quarry, which is located in a mountainous area near Athens and which has been exploited in the past for cement production, was chosen for bioremediation experiments in collaboration with the quarry owners, TITAN Cement Co. Three different areas within the quarry were selected representing 3 different topographies. These were a compacted, flat area with seasonal water logging (untreated control), a gently sloping, land formed area; and artificially created mounds of overburden rock material formed on the flat area. Compost produced from mixing municipal waste, green waste and digested sewage sludge at the recycling plant of the Association of Communities and Municipalities in the Attica Region (ACMAR), near Athens was used for nutrient amendment. Six replicates of three treatments were applied with varying compost application rates in order to examine the effect of the addition of organic matter in the establishment of suitable vegetation on the infertile schist material. Pine trees (Pinus halepensis Mill.), 4 per replicate, were pocket planted in a volume of 3 liters of schist or schist-compost mixture (1:2 and 2:1). Tree height, main stem diameter and branching were measured following planting and these measurements will be repeated annually to test hypotheses that compaction and water logging reduce Pine establishment by natural regeneration and that nutrient addition improves establishment of pine on schist waste. The availability of nutrients and variation in other soil physico-chemical parameters will also be studied in each treatment and related to plant growth. As a demonstration project, open days are planned on the site for the public and official bodies involved in municipal waste handling and other relevant areas. The aim of these will be to illustrate the effective re-use of waste for the sustainable restoration of post-industrial and degraded land.

Jan 1, 2006

Bioremediation of Acid Mine Water Using Fly Ash and Desulfotomaculum nigrificans One of the major concerns for the mining industry especially after the mine closure is the generation of acid mine drainage (AMD), and its discharge into the environment surrounding the abandoned mines, leading to serious environmental pollution. AMD is usually characterized by high concentration of dissolved metal ions, sulfate and low pH. The efficacy of fly ash was assessed for the removal of metal ions such as iron, zinc and copper as well as sulfate from synthetic acid mine water. Complete removal of ferric and ferrous, 80 per cent of Zn, 83 per cent of Cu and 62 per cent sulfate removal could be achieved, with a concomitant increase in the pH value from 2.3 to 7 using fly ash as an adsorbent under appropriate conditions. The free energy of adsorption of the metal ions onto fly ash was found to be negative, while the adsorption process was endothermic and adhered to the Lagergren pseudo-first order rate equation. Bioremediation studies using a typical sulfate reducing bacterium namely Desulfotomaculum nigrificans, after pre-treatment of the synthetic acid mine water with fly ash, cumulatively resulted in about 94 per cent zinc, 100 per cent copper and 90 per cent sulfate removal. The possible mechanisms of metal ion removal and sulfate reduction from acid mine water utilising Desulfotomaculum nigrificans are discussed.

Sep 13, 2010

By Evvie Chockalingam

Acid mine drainage (AMD) is a perennial problem particularly in abandoned mines, occurring due to the oxidation of sulphidic mine wastes. AMD is usually characterized by high concentration of dissolved metal ions, sulphate and low pH. The efficacies of substrates such as rice husk and the bark of Eucalyptus tereticornis (Sm.) are assessed for the removal of metal ions such as iron, zinc and copper from acid mine water obtained from an abandoned copper mine. Over 99 % of Fe, 98 % of Zn and 95 % of Cu removal could be achieved from acid mine water, with a concomitant increase in the pH value by three units using rice husk as an adsorbent. With respect to tree bark as an adsor-bent, about 96 % of Fe, 75 % of Zn and 92 % of Cu could be removed from the acid mine water with an attendant increase in the pH value by two units. The free energy of adsorption of the metal ions onto rice husk and tree bark is found to be negative in both the cases, while the adsorption process is endothermic in the case of rice husk and exothermic for tree bark. The adsorption process is found to adhere to the Lagergren pseudo-first order rate equation. The reduction of sulphate con-centration in the acid mine water is achieved using a typical sulphate reducing bacteria namely De-sulfotomaculum nigrificans. Desorption experiments indicate that about 88 % of Zn, 87% of Cu and over 77 % of Fe could be recovered from rice husk and over 70 % of Fe, 82 % of Zn and 77 % of Cu could be desorbed from the metal loaded tree bark using 5 M HCl. The possible mechanisms of metal ion and sulphate removal from acid mine water using rice husk and tree bark are discussed.

Jan 1, 2014

By S. Vimalnath

The bioremediation capability of Pseudomonas aeruginosa cells was investigated for the biosorption of lead ions from aqueous solution. The various parameters such as pH, kinetics, biomass loading and concentration of lead affecting the biosorption process were studied. Complete bio removal of lead could be achieved for an initial concentration of 12.4 mg/L at pH 6.2, corresponding to typical industrial effluents. The biosorption isotherm followed Langmuirian behavior and the Gibbs free energy (?G) was determined to be-30.8 kJ/mole, indicative of chemisorption between the functional groups of the bacterial surface and lead. The biosorption kinetics adhered to a pseudo-second order model. The possible mechanisms of interaction of lead with the bacterial cells are discussed. A carbon paste electrode with a coating of biomass was developed for lead ion detection in aqueous solution using cyclic voltammetric technique. Concentrations of lead up to 5x10-5 M could be successfully detected using cyclic voltammetry, which could be improved to a detection limit of 1x10-8 M adopting differential pulse anodic stripping voltammetry (DPASV). Keywords: Pseudomonas aeruginosa, bioremediation, lead, voltammetry

Sep 1, 2012

By S. Carsten, S. Subramanian, R. Vasant Kumar, S. Vimalnath

"The effect of EPS components namely protein, polysaccharide and biosurfactant purified from Pseudomonas fluorescens was investigated for the bioremediation of Pb(II) ions in aqueous solution. Two parameters, namely the amount of EPS component and the concentration of Pb(II) ions were optimized for maximum Pb(II) ions uptake. The EPS components interacted with Pb(II) ions were characterized using energy dispersive X-ray analysis (EDAX) to ascertain the presence of Pb and by attenuated total reflection-Fourier transform infrared spectroscopy (ATR–FTIR) to reveal the functional groups involved in Pb(II) binding. The maximum Pb(II) binding capacities of protein, polysaccharide and biosurfactant were found to be 11.4, 0.55 and 0.11 mg mg-1 respectively. The biosorption processes followed the Langmuir model. The Gibbs free energy (?G0) values of -23.6, -20.9 and -23.2 kJ mol-1 for Pb(II) biosorption by protein, polysaccharide and biosurfactant respectively, attested to spontaneous, chemisorption processes. The ATR–FTIR results revealed the presence and involvement of amino, hydroxyl, carboxyl, phosphate, and methyl functional groups in Pb(II) binding and EDAX analysis confirmed the interaction of Pb with EPS components. The Pb(II) binding mechanisms of EPS have been discussed. The results demonstrate the potential of the purified EPS components of P. fluorescens for removal of Pb(II) ions from aqueous solution INTRODUCTION Waste water containing toxic metals from various industries pose a serious threat to human health and ecosystem. For treating such waste water, conventional methods like chemical precipitation, coagulation, evaporative recovery, electrodialysis, floatation, flocculation, ion exchange, reverse osmosis, nanofiltration, ultrafiltration are used (Lakherwal et al., 2014). These methods have some disadvantages like toxic sludge generation, incomplete metal removal and high cost (Singh and Gadi, 2012), necessitating the search for cheaper and efficient alternative methods. Bioremediation is one of the emerging alternatives where bacteria, fungi, yeast, algae and other biological substances are used as sorbents to remove toxic heavy metals (Vijayaraghavan, et al., 2015). Recently, the bioremediation potential of extracellular polymeric substances (EPS) of microbial origin has attracted attention. However, for Pb(II) removal, there is a paucity of literature focusing on EPS."

Jan 1, 2018

By Stoyan N. Groudev, Ioannis Paspaliaris, Irena I. Spasova, Kostas Komnitsas

"Acid mine drainage waters generated in the uranium deposit Curilo, western Bulgaria, were treated by means of a constructed wetland located in the deposit. The waters had a pH in the range of about 2.5 - 4.1 and contained uranium, radium, some heavy metals (iron, manganese, copper, zinc, cadmium, cobalt, nickel, lead), arsenic and sulphates in concentrations much higher than the relevant permissible levels for waters intended for use in the agriculture and/or industry. The wetland covered an area of about 140 m2 and was characterized by an abundant water and emergent vegetation and a diverse microflora. Typha latifolia, Typha angustifolia and Phragmites australis were the prevalent plant species in the wetland but species of the genera Juncus, Eleocharis, Scirpus, Carex and Poa as well as different algae were also well present.The water treatment was started in July 2001 and until now (August 2002) the wetland effluents were characterized by pollutants concentrations decreased below the relevant permissible levels. The water flow rate through the wetland during this period varied in the range of about 0.2 - 1.0 l/s. The water clean-up markedly depended on the temperature but was efficient even in the cold winter months at air and water temperatures close to 0oC. The removal of pollutants was due to different processes but the microbial dissimilatory sulphate reduction and the sorption of pollutants on organic matter (viable and dead plant and microbial biomass) and clay minerals played the main role. Negative effects of the pollutants on the growth and activity of the indigenous plant and microbial communities were not observed."

Jan 1, 2003

By Stoyan N. Groudev, Ioannis Paspaliaris, Plamen S. Georgiev, Irena I. Spasova, Kostas Komnitsas

"The uranium deposit Curilo located in Western Bulgaria for a long period of time was a site of intensive mining activities including both the open-pit and underground mining techniques as well as in situ leaching of uranium. The ore was rich in pyrite and, apart from uranium, contained some non-ferrous metals. The mining operations in the deposit were ended in 1990 but since that time the deposit is a permanent source of acid drainage waters. The generation of these waters is connected mainly with the oxidation of pyrite and other sulphide minerals by the indigenous acidophilic chemolithotrophic bacteria.Laboratory and pilot-scale studies revealed that an efficient clean-up of the above-mentioned waters was achieved by means of passive systems of the wetland type. In 2001 a wetland was constructed in the deposit to treat a portion of the acid drainage waters which were discharged to a small ravine. This ravine reaches into the Teina, a small river that is a tributary of the upper reaches of the Iskar river.The wetland was constructed in the ravine and its bottom was located on intrusive rocks with a very low permeability. The rocks were covered by a 30 – 50 cm layer consisting of a mixture of soil, plant compost and crushed limestone. The wetland was characterized by an abundant water and emergent vegetation and a diverse microflora. Typha latifolia, Typha angustifolia and Phragmites australis were the prevalent plant species in the wetland but species of the genera Juncus, Eleocharis, Carex and Poa as well as different algae were also well present.The acid drainage waters treated by the wetland had a pH in the range of about 2.5 – 4.1 and contained uranium, radium, some heavy metals (iron, manganese, copper, zinc, cadmium, lead), arsenic and sulphates in concentrations much higher than the relevant permissible levels for waters intended for use in the agriculture and/or industry. The watercourse through the wetland covered a distance of about 0.2 – 1.0 l/s."

Jan 1, 2003

By D. Raghavan

In recent years, the environmental issues related to the landfill shortage received considerable importance. Many polymeric solid wastes have high resistivity to chemical and biological degradation, and options are sought by which these wastes can be recycled and reused, or reduced in volume. The present study investigated the biodegradation of polyethylene by Aspergillus niger, and biodesulfurization of rubber by pure and mixed cultures of Thiobacillus thiooxidans and Thiobacillus ferrooxidans. The fungus, A. niger, was found to metabolize portions of polyethylene. Quantitative calorimetric measurements revealed that the amorphocity of the sample decreased during biodegradation. Removal of sulfur from synthetic rubber samples containing 2 to 15% sulfur, was studied in shake flask experiments and the associated oxygen consumption was studied in Warburg respirometers. The mixed bacterial cultures were more effective in the desulfurization of rubber samples than the pure cultures of T. thiooxidans and T. ferrooxidans. The data obtained in these studies may have far reaching consequence for the development of new effective processes regarding the remediation and management of discarded polymeric packaging wastes and car tire rubber materials.

Jan 1, 1993

Mispah form (FAO: Lithosol) soil contaminated with >380 000 mg kg-1 creosote was co-composted with sewage sludge and poultry manure for a period of 19 months. The soil was mixed in a ratio of 1:1 (v/v) with wood chips to improve aeration. The soil-wood chips mixture was then mixed in a ratio of four soil-wood chips mixture: one compost material (sewage sludge or poultry manure). Compost heaps were set up in triplicate on wood palettes overlaid with double layers of nylon straw bags. Control experiments, which contained the contaminated soil-wood chips mixture without sewage sludge or poultry manure were set up in triplicate. Moisture, temperature, pH, ash content, C:N ratio of the compost and the creosote concentration of the soil was monitored monthly. The concentration of selected creosote components in the soil was also determined at the end of the incubation period. Temperature was observed to rise to about 60¦C in the poultry manure and about 58¦C in the sewage sludge within two months of incubation, while temperature in the control fluctuated between 15¦C and 35¦C throughout the incubation period. Creosote concentration in the control was reduced by 17 per cent at the end of the incubation period while concentration of creosote in the sewage sludge and poultry manure compost was reduced by about 99 per cent. The percentage rate of creosote reduction in the poultry manure compost was initially lower than observed in the sewage sludge compost. However, the reduction rate became similar towards the end of the treatment period. The concentrations of selected creosote components were reduced by about 100 per cent within the same period. Respiration of compost microorganisms and counts of microbial populations showed that microbial activity correlated with reduction in creosote concentration.

Jan 1, 2004

By J. S. S. Allwin Ebinesar

The bioremediation of zinc was carried out using Pseudomonas alcaligenes, a Gram negative heterotroph. The parameters such as pH, bacterial cell number, initial metal concentration, and interaction time on the biosorption of zinc were studied. The maximum zinc uptake was found to be about 95% at pH 5 and biomass loading of 2.7x1010cells/mL. The biosorption isotherm for zinc exhibited Langmuirian behavior and the Gibbs free energy of biosorption was determined to be -31.2 kJ/mol, indicative of coordination of zinc with the functional groups on the bacterial surface. The adsorption kinetics followed a pseudo-second order equation. FTIR and EDAX studies confirmed the interaction of zinc with the biomass. The mechanisms of metal-microbe interactions are discussed.

Sep 1, 2012

As mining and extraction processes improve and lower grade ores become economic, mining for most ores have extended to depths beneath the zone of weathering oxidation created over geological history. These deeper openings expose sulphidic formations and reduced mineral species as either primary ore or simply as accessory or gangue minerals in the country rock. This is not new, underground mining for primary base metal and gold have long been familiar with these problems, as have coal and uranium miners. The change is simply that the number of mines exposing sulphidic waste rocks, tailings to the atmosphere and/or leaving behind dewatered sulphidic rock surrounding the mine void has increased the number of sites from where acid mine drainage (AMD) may emanate with consequential problems for the hydrological environment including downstream catchments (Miller et al, 1996). The principles of ecologically sustainable development (Brundtland, 1987) have been more or less ratified by all the developed nations of the world and this has led, amongst other things, to an expansion unprecedented in the research and documentation of means for achieving long-term environmentally acceptable stabilisation of solid wastes of all sorts. These have revealed that AMD problems can be minimised and even reversed in many cases by the integration or co-disposal of biodegradable organic wastes with oxidising sulphidic wastes (Elliott et al, 1996). This paper considers how this might be achieved at mine sites in Australia and the results encountered at some relevant sites.

Jan 1, 1997

By Daniela Bustinza

En el presente proyecto de investigación evalúa la posible reducción progresiva de acuíferos contaminados con uranio, mediante la estimulación de microorganismos reductores de metales disimiladores, los electrodos de estos organismos se comportan como donadores de electrones y de igual forma como aceptador de electrones que se llaman "electroquímicamente estimulantes” generalmente, estas bacterias reducen los minerales insolubles que contienen iones metálicos oxidados para poder generar energía. El organismo que se utiliza es el Geobacter este organismo puede desarrollar biobarreras permeables para la inmovilización de uranio a largo plazo.

Sep 30, 2022

By Melitza Cornejo

La lixiviación con cianuro es el proceso utilizado por la industria minera para extraer oro y plata. El cianuro es un compuesto tóxico para la mayoría de los organismos vivos. Sin embargo, muchos microorganismos son capaces de tolerarlo e incluso degradarlo. En ese sentido, el texto caracteriza y analiza la composición de las comunidades microbianas de suelos y aguas contaminadas por residuos de cianuro, producto de la actividad minera en la región La Libertad a través de la metagenómica dirigida al ADNr. De forma que se logró aislar e identificar molecularmente 89 cepas microbianas, como los géneros Pseudomonas, Bacillus y Alcaligenes. Asimismo, la capacidad de degradación del cianuro ha sido establecida en base a pruebas in vitro considerando estas cepas, ya sea individualmente o en consorcios.

Jan 1, 2017

By Janine Acasi, Robert D. Rogers, Jonathan K. Trautman, Richard C. Cronn, James H. Wolfram

The demand for phosphate will continue into the future, because phosphate is currently irreplaceable as a plant nutrient and in many chemical applications. Phosphate is not recycled, so the supply must be replenished through a process of mining and purification. The most energy intensive part of the process, separation of phosphate from the ore, requires 54 to 78% (depending on the method used) of the estimated 0.5 quads of energy consumed by the U.S. phosphate industry. This paper provides data from a project designed to determine if a biotechnology can be developed to separate phosphate from its ore. Several hundred microorganisms (both bacteria and fungi) capable of solubilization of phosphate ore have been isolated from the environment. Work is ongoing to determine the mechanism for solubilization. In addition, how these organisms could be used in a bioprocess is being investigated.

Jan 1, 1989

By C. Xiao, R. Chi

In recent years, the biosolubilization of rock phosphates by phosphate-solubilizing microorganisms has become a major topic of research studies. However, the microorganisms used in previous studies were mostly single and known species, and studies seldom focused on mixed communities of microorganisms or microbial consortia from natural environments. In this study, a microbial consortium from rhizospheric soil was used to solubilize two different compositions of medium- to low-grade rock phosphates — designated as ore samples 1 and 2 — in a laboratory-scale bioreactor. The microbial consortium showed good growth in the bioreactor, reduced the suspension pH and released soluble phosphates from the ores. Scanning electron microscopy with energy-dispersive X-ray spectroscopy showed the action of the microbial consortium on the ore surface. There was considerable corrosion on the ore surface, and 3.42 and 4.93 percent of the element phosphorus was removed from ore samples 1 and 2, respectively. Microorganisms were sampled from the bioreactor for the analysis of their community structures by Illumina MiSeq high-throughput sequencing. The analysis show that the suspensions added with different ore samples had different microbial diversities and richness. This study provides new insight into the biosolubilization of rock phosphates by a microbial consortium directly taken from rhizospheric soils.

By Gabriela Huamán

La bioosorción es un proceso de formación de metales pesados, basado en la actividad química de una biomasa, cuya tecnología se convierte en una de las más promisorias alternativas para el tratamiento de efluentes que contienen metales pesados en bajas concentraciones. Esta tecnología utiliza diferentes tipos de biomasas como: algas, hongos, levaduras, bacterias, microorganismos y sub-productos agrícolas. En este sentido, el presente texto describe las ventajas de reutilizar dicha biomasa, tales como el bajo costo de operación y la elevada capacidad de remoción de metales pesados, sobre los métodos de remoción convencionales.

Sep 14, 2009

By Ting Luo, Hongmin Zhu, Kai Huang, Shuanglong Du, Tao Gui, Yifan Xiu

"Poplar cotton, a cellulosic material, was found to adsorb metals ions effectively. In the present study, the biosorption characteristics of Pb(II) onto poplar cotton, were evaluated as a function of pH, contact time, biomass dosage, initial concentration and temperature. Within the pH range investigated (1.0-6.0), the optimal pH value suitable for the uptake of Pb(II) was determined. The maximum adsorption capacity obtained from the Langmuir model is around 0.52mol kg for the poplar cotton sorbent. Equilibration is achieved in 60min for the studied concentrations. Results suggest that the poplar cotton can be directly used as an effective and low-cost biosorbent for removal of Pb(II) from aqueous solution.IntroductionHigh value utilization of various biowastes produced in agricultural, forestry, fishing industries, etc. have attracted great interest from many researchers [1], Biomass, due to its easy availability, great abundance, low cost, easy processing, eco-friendliness and biodegradability, has exhibited exciting potential for being used as raw materials for the preparation of many new hi-tech functional products [2]. For example, using biowastes to synthesize sorbents for application in the removal of toxic substances such as dyes [3], heavy metals from the waste water [4] or in the recovery of valuable metals [5] from the ore tailings and secondary metals resources has made great progress in last decade. In present study, poplar cotton, a kind of white light fluff of poplar trees, like that of dandelion, usually flying in the air each spring was tested as a biosorbent for the remove lead ions from the water. It may also provide some good ideas for new sorbent design. Lead, as one of the typical poisonous heavy metals, for its typical toxicity and extensive pollution, was chosen to test the adsorption behavior of poplar cotton in aqueous solutions."

Jan 1, 2012

By M. L. Apel

A conceptual approach to the bioremediation of uranium mill tailings (UMT) is presented., This approach focuses on, biosorption to remediate the. effluent solution and bioleaching remediate the. solid fraction. Comparative biosorption studies were performed with synthetic single metal solutions using a commercially available biopolymer. The maximum rate of metal sorption by calcium alginate was found to be 5.43, 6,39, 6.48, and 9.74 mg dm-3 min-1 for U022*, Ba2+, Cd2+ and Zn2+, respectively. Activation energy (AEa) for barium uptake by calcium alginate beads was determined to be 11.5 kJ, indicating a diffusion controlled reaction mechanism. Thermodynamic values (AG+ AH+ and ASt) of the metal-alginate activated complex, as reported in the literature, were found to be in agreement with those calculated from this research. Results show that this biopolymer can be effectively applied in the removal of select metals prevalent in uranium mill tailing effluents and associated waste streams.

Jan 1, 1991